mouse lncrna expression microarray v3.0 (Arraystar inc)
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Mouse Lncrna Expression Microarray V3.0, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
Images
1) Product Images from "Propofol Alters Long Non-Coding RNA Profiles in the Neonatal Mouse Hippocampus: Implication of Novel Mechanisms in Anesthetic-Induced Developmental Neurotoxicity"
Article Title: Propofol Alters Long Non-Coding RNA Profiles in the Neonatal Mouse Hippocampus: Implication of Novel Mechanisms in Anesthetic-Induced Developmental Neurotoxicity
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
doi: 10.1159/000493875
Figure Legend Snippet: Propofol alters messenger RNA (mRNA) profiles in the mouse hippocampus. (A) The images of denaturing agarose gel electrophoresis of total RNAs isolated from 4 intralipid- or 4 propofol-treated mouse hippocampal tissues. (B) The box plots display similar distributions of normalized mRNA intensity values across 4 control and 4 propofol samples, suggesting that the microarray data can be used for further analysis. Each box plot consists of a box with a central line and two tails. The central line represents the median of mRNA intensity values whereas the tails represent the upper and lower quartiles. (C) Heatmap showing profiles and the hierarchical clustering of differentially expressed mRNAs between intralipid- and propofol-treated mouse hippocampus (n=4; p<0.05). The red signal refers to high relative expression and the green signal referred to low relative expression.
Techniques Used: Agarose Gel Electrophoresis, Isolation, Control, Microarray, Expressing
Figure Legend Snippet: Propofol alters profiles of long non-coding RNAs (lncRNAs) in the mouse hippocampus. (A) The box plots showing the similar distributions of normalized lncRNA intensity values from 4 control and 4 propofol samples. Each box plot consists of a box with a central line and two tails. The central line represents the median of lncRNA intensity values, whereas the tails represent the upper and lower quartiles. (B) The volcano plot illustrating the differentially expressed lncRNAs (red dots) between control and propofol groups. These altered lncRNAs were either downregulated (left red dots) or upregulated (right red dots) following propofol exposure. (C) Heat map showing the profiles and hierarchical clustering of differentially expressed lncRNAs in intralipid- and propofol-treated mouse hippocampus (n=4; p<0.05). The red signals refer to high relative expression and the green signals indicate low relative expression. Among 24,881 lncRNAs analyzed, 159 lncRNAs were differentially expressed following propofol exposure. (D) Pie chart showing the ratio of various types of dysregulated lncRNAs. Specifically, over 50% total dysregulated lncRNAs were intergenic lncRNAs.
Techniques Used: Control, Expressing
Figure Legend Snippet: Propofol-induced individual dysregulated lncRNAs and their neighbor protein-coding genes which are also altered following propofol exposure. Note: chr is the abbreviated chromosome. P value and fold change is shown as propofol vs. control. Expression changes are shown compared with control group
Techniques Used: Control, Expressing
Figure Legend Snippet: Bioinformatics analysis of neuro-degenerative signaling pathways of top 50-ranked altered lncRNAs. The green and red arrows, respectively, representative lncRNA downregulation and upregulation following propofol exposure.
Techniques Used: Protein-Protein interactions